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黃麴毒素的分析；採用SPE-HPCL更快速  免疫親合管與SPE固相萃取管之操作步驟的比較


                                                               SPE幫你節省10倍的時間
 前言
                                                               比免疫親合管再現性更好
 傳統的黃麴毒素純化分析是採用免疫親和柱 IAC，需要採用”Binding and Wash”的機制，來濃縮和純化黃麴
 霉毒素，純化後的樣品隨後用於分析。固相萃取管 (SPE)，則是一種新的替代方法，利用“Interference
                                                               The following is an experiment comparing the use of the Supel
 Removal”的方法達到樣品純化的目的，並且大大縮短樣品處理的時間。                           Tox AflaZea SPE cartridge to a leading immunoaffinity column
                                                               for the analysis of aflatoxins B1, B2, G1 and G2 in peanut paste.
                                                               Theresults illustrate Supel Tox AflaZea SPE cartridges are   食品檢測分析
 Supel Tox AflaZea SPE Cartridge  更快速; 再現性更好                   superiorto immunoaffinity columns in terms of process simplicity,
                                                               timerequired for sample preparation, and control of variation.

 Features and Benefits
                                             免疫親合管                                     SPE 固相萃取管
  有效去除黴菌毒素的干擾; 用於後續分析
           樣品製備時間           6    0  m  n i  a s   8   ;  m  d / s e l p  y a    6  m  n i  8   ;  a s   0  m  d / s e l p  y a
  比免疫親合管再現性更好
                            • 需要大量的液體製備                                      • 只需少量的液體製備
 比免疫親合管節省10倍的時間            • 需要控制流速                                         • 需要抽氣裝置 Vacuum filtration
             使用方式           • 需要較多的步驟                                        • 操驟步驟較少
 方法與部驟非常簡單
                            • 需要配製鹽類緩衝液                                      • 不需要配製額外的緩衝鹽類
 室溫保存; 效期比免疫親合管更久          • 需要冷藏保存                                         • SPE管室溫保存即可
                            Stage 1 (15 minutes)                             Purify and Transfer (6 minutes)
 製備時間短；再現性更好 的固相萃取管         1. Configure manifold for waste collection       1. Configure manifold for sample
                            2. Add 1 mL sample to 17 mL of                   collection
 ●………………………………………………………………………………………………....●
                              phosphate buffered saline, vortex              2. Mount cartridges
 ”Binding and Wash”與“Interference Removal” 方法比較  3. Uncap/mount/drain cartridges by gravity  3. Load 2 mL sample
                            4. Apply reservoirs, load sample onto cartridges  4. Elute and collect under vacuum
                            Stage 2 (15 minutes)                             5. Transfer 0.2 mL sample to vial
                            5. Rinse interferences                           6. Dilute sample and vortex Analysis
             操作步驟
                            6. Reconfigure manifold for sample collection    Analysis
                            7. Elute/collect sample
                            Stage 3 (30 minutes)
                            8. Evaporate sample to dryness
                            9. Reconstitute sample and vortex
                            10. Transfer 0.2 mL sample to vial
                            11. Dilute sample and vortex
                            Analysis


      ●………………………………………………………………………………………………....●
         SPE Product list

              S P  C   E  d i r t r a  g e  P  e i f i r u  T   d  g r a  t e  S a m  e l p  M  h t e  o d  C    . t a  N  . O

                                                          Grains, feeds, TMR samples,
             Supel Tox AflaZea   Aflatoxin and Zearalenone  peanuts,peanut products and                 55314-U
                                                          aqueous solutions            Interference
                                                                                       removal
              S u p  l e  T o x  D O N  D e o  y x  e l a v i n  n  ( l o  D O N )  W h e  o l f   , t a  a   r u  n  c   d  n r o  5 5 3 1  - 6  U
             Supel Tox Tricho   Trichothecenes (Type A  na  ) B   d  G  a   s n i a r  n  c   d  o m    x e l p  m  s e c i r t a  5 5 3 0  - 8  U
                                                          Grains, feeds and other complex
            Supel Tox TrichoBind  Trichothecenes (Type A and B)                                         55307-U
                                                          matrices
                                                                                       Multifunctional
           S u p  l e  T o x  F u m o  n i B i n  d  F u m o  s n i s i n  B (  a   1  n  B   d  ) 2  G  a   s n i a r  n  e r e c   d  s l a  bind and elute  5 5 3 1  - 5  U
            Supel Tox OchraBind       O c   o t a r h  n i x  A  G  a   n i a r  n  e f   d  e  a s   d  m  s e l p  5 5 3 1  - 8  U

         References
         Cornell University Department of Animal Science Site. http://www.ansci.cornell.edu/plants/toxicagents/aflatoxin/aflatoxin.html (accessed Feb 2013
         World Health Organization International Agency for Research on Cancer Site. http://monographs.iarc.fr/ENG/Classification/index.php (accessed Feb 2013).
         World Health Organization International Agency for Research on Cancer Site. http://monographs.iarc.fr/ENG/Monographs/vol82/volume82.pdf (accessed Feb 2013)
         US Food and Drug Administration Site. http://www.fda.gov/ICECI/ComplianceManuals/ CompliancePolicyGuidanceManual/ucm074703.htm (accessed Feb 2013)


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