Page 12 - 2016 July 專刊-H-0722

【Apoptotic DNA Ladder Kit】 ROC-11835246001

偵測原理與標的 實驗的操作流程

偵測 利用 Agarose 核酸電泳方式，偵測
凋亡細胞中的LMW DNA核酸片段，
該套組利用玻璃纖維 column，可快
速分離出細胞的核酸，直接進行核
的斷裂 酸電泳之分析。
DNA

適用的實驗分析方法
--Agarose 核酸電泳

適用的偵測檢體
--培養的細胞 (懸浮or貼附)
--全血細胞

產品優點
• 製備時間短；只需 20 分鐘；就可
以純化出核酸片段
• DNA 萃取免用有機溶劑
• 套組內有提供 Postive control
(LMW DNA核酸片段)，可以當
作 Apoptotic sample 的對照組

Sample Sample volume Yield of purified DNA

W h o l b e l o o c d s l l e C e r u t l u c d ( s l l e K 5 6 ) 2 3 ~ 6 u g
C e r u t l u c d s l l e 2 X 1 0 8 1 0 u g

DNA ladder assayed with the Apoptotic DNA Ladder Kit
M = Size marker
Result
– = Control cells without camptothecin
+ = Cells treated with camptothecin
C = Positive control from the kit

References
1. Carrageenan Induces Cell Cycle Arrest in Human Intestinal Epithelial Cells in Vitro Sumit Bhattacharyya, Alip Borthakur,
Pradeep K. Dudeja, and Joanne K. Tobacman J. Nutr., Mar 2008; 138: 469 - 475
2. Assessment of Fat Specific Protein 27 (Fsp27) in the Adipocyte Lineage Suggests a Dual Role for Fsp27 in Adipocyte
Metabolism and Cell Death Ji Young Kim, Kun Liu, Shengli Zhou, Kristin Tillison, Yu Wu, and Cynthia M. Smas Am J
Physiol
Endocrinol Metab, Jan 2008; 10.1152/ajpendo.00104.2007.
3. Glutathione Levels Modulate Domoic Acid–Induced Apoptosis in Mouse Cerebellar Granule Cells Gennaro Giordano,
Collin C. White, Isaac Mohar, Terrance J. Kavanagh, and Lucio G. Costa Toxicol. Sci., Dec 2007; 100: 433 - 444.

UNI-ONWARD 10