Page 14 - 2016 July 專刊-H-0722

【 In Situ Cell Death Detection Kit, Fluorescein TUNEL assay】
ROC-11684795910 / ROC-12156792910

偵測原理與標的
偵測
利用帶有螢光分子之 Fuorescein-dUTP 或 TMR-
dUTP去標定單一顆細胞 genomic DNA 之3’OH。
藉由TdT (terminal transferase) 酵素反應；能將
的斷裂 帶有螢光的核苷酸接到斷裂的 DNA 3 端的 OH
DNA
group 上；再利用螢光顯微鏡或是流式細胞儀，
即可進行影像觀察。

適用的實驗分析方法
--Flow/ 螢光顯微鏡

適用的偵測檢體
--培養的細胞 (懸浮or 貼附)
--冷凍切片和石蠟包埋切片

產品優點
• 可作單一顆細胞的分析
• 高敏感度低背景值
• 操作簡單，時間只需 2 小時

Detection of apoptotic cells by flow cytometry using the In
Situ Cell Death Detection Kit, Fluorescein.
HL60 cells were cultured in the absence (A) or presence
(B) of 2 μg/ml Camptothecin for 3 h at 37°C.
Cells were incubated either with TUNEL reaction mixture
( ) or label solution as negative control ( ) or
PBS for autofluorescence ( ).

Detection of apoptotic cells (green) by Cell suspension stained with the In Use of the In Situ Cell Death Detection Kit,
fluorescence microscopy in a tissue Situ Cell Death Detection Kit, TMR red to detect apoptotic cells (red) by
section from rat. A tissue section from a Fluorescein. U-937 cells induced with IHC staining.
rat spinal cord was prepared and 4 μg/ml camptothecin show positive
assayed with the In Situ Cell Death staining of apoptotic nuclei.
Detection Kit, Fluorescein.

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