Page 7 - 2015 April 3D 細胞培養新趨勢

n3D 磁浮培養系統 Scaffold free format

n3D的磁浮培養系統; 利用了奈米磁珠的特性; 讓細胞
與奈米磁珠結合後; 使細胞帶有磁性。因此只要在培養皿 3D Cell Culture

的上蓋放置磁鐵; 細胞就能因為磁力而聚集在一起: 進而形
成球體的細胞團塊; 生長出3D的結構。

利用3D 磁浮培養; 可觀察細胞的生長曲線或是進行藥物篩選試驗

Fig8. Viability of PC3 spheroids of various sizes as measured by (left) the Real-time CellTiter-Glo assay

3D 磁浮培養系統的優點
提供具有生物相容性的奈米磁珠; 使細胞帶有磁性

可以使細胞長成3D的球狀結構; 更接近於真實體內的環境
操作簡單; 節省時間; 適合high throughput 的操作
不需要特別的儀器; 即可操作

3D 磁浮培養適用的細胞

Cell lines Primary cells
• Murine Endothelial • Human Pulmonary Microvascular Endothelial Cells
• Murine Embryonic Fibroblasts, pre-adipocytes (3T3) (HPMEC)
• Murine Adipocyte • Human Tracheal Smooth Muscle Cells (HTSMC)
• Murine Melanoma • Human Small Airway Epithelial Cells (HSAEpiC)
• Murine Neural Stem Cells • Human Pulmonary Fibroblasts (HPF)
• Rat Hepatoma • Human Mesenchymal Stem Cells (HMSC)
• Human Astrocytes • Human Bone Marrow Endothelial Cells (HBMEC)
• Human Glioblastoma Multiforme (GBM) LN 229 • Human Umbilical Vein Endothelial Cells (HUVEC)
• Human Embryonic Kidney 293 (HEK 293) • Human Aortic Vascular Smooth Muscle (HASMC)
• Rat Vascular Smooth Muscle (A10) • Murine Chondrocytes
• Human Hepatocellular Carcinoma Cells (HepG2)
• Human Lung Adenocarcinoma Cells (A549)
• Human Colorectal Carcinoma Cells (HCT 116)
• Human Pancreatic Epithelioid Carcinoma (PANC-1)

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