Page 17 - 2015 April 3D 細胞培養新趨勢

3D CelluSponge 產品分類與適合的細胞株

產品 CelluSponge CelluSponge-Galactose CelluSponge-Collagen

HPC scaffold with no Galactose conjugated HPC Collagen conjugated
特性
bioligand conjugation scaffold HPC scaffold
由羥丙基纖維素(HPC) 製 適用於需要半乳糖的細胞， 適合需要膠原蛋白基質的
成，適用於不需要特定 例如: 肝細胞在體外之培養， 細胞培養。例如: 幹細胞、 3D Cell Culture
Ligand 的細胞，如一般細 藥物檢測與藥物開發中早期 間質幹細胞(MSCs) 、神
適合的用途
胞與癌細胞株。 篩選使用。 播種1天後細胞 經細胞培養。
形成3D功能性的球體，此球
體可保持穩定100天。

• Human breast cancer • Primary hepatocytes (rat, • Embryonic stem cells
cells (MCF-7) human and monkey) • Human mesenchymal
• Mouse embryonic • Hepatic cell lines (Huh 7, stem cells
適合細胞株
fibroblasts (NIH-3T3) Huh 7.5, HepG2 and hepatic • iPS derived
• Human foreskin progenitor) cardiomyocytes
fibroblast (HFF) • Neuronal cells

A B NIH3T3 cells 5 days post-
seeding

(A) and B): SEM images of NIH-
3T3 cultured on day 1 at low
(scale bar 100 μm) and high
magnification (scale bar 10 μm),
C D respectively
(C) and D): SEM images on day
5 at low (scale bar 100 μm) and
high magnification (scale bar 10
μm), respectively.

SEM images of primary rat hepatocyte spheroids formed in 3D CelluSponge-GAL.

SEM images of neural differentiation of human mesenchymal stem cells on 3D CelluSponge-COL at different
time points of differentiation. The time points are as follows: (A) 2 days (B) 7 days and (C) 14 days. The red
arrow indicates the cell body, while the green arrows indicate the neurite. Scale bar 30 μm.

15 UNI-ONWARD